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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry - domestic rabbit monoclonal (JM30-30)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of GABARAP on different lysates with Rabbit anti-GABARAP antibody (ET1705-53) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 14 kDa Observed band size: 16 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GABARAP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse kidney tissue lysate Lane 2: Rat liver tissue lysate
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
gene information - rat Gabarapl1
- synonym:Gec1
- description:GABA type A receptor associated protein like 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Gabarapl1 antibody
- Gabarapl1 ELISA/assay
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