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- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 4 - domestic rabbit polyclonal (SD20-42)
reactivity: human, mouse, rat, dogs, bovine
application: immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: ELISA - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: ELISA - domestic rabbit monoclonal (JB35-53)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
All lanes: Western blot analysis of FURIN with anti-Furin antibody [JB35-53] (ET7107-37) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate. Lane 2: FURIN knockdown Hela whole cell lysate. ET7107-37 was shown to specifically react with FURIN in wild-type Hela cells. Weakened band was observed when FURIN knockdown samples were tested. Wild-type and FURIN knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-FURIN antibody (ET7107-37, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Western blot analysis of Furin on different lysates with Rabbit anti-Furin antibody (ET7107-37) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: Ramos cell lysate (low expression) Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-37) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - rat Furin
- synonym:Pace; Pcsk3
- description:furin (paired basic amino acid cleaving enzyme)
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- Furin antibody
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