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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
citations: 1
Western blot analysis of Mouse, Rat Liver cell lysates showing detection of ~17.2 kDa FUNDC1 protein using Rabbit Anti-FUNDC1 Polyclonal Antibody (SPC-638). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Lane 3: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody (SPC-638) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~17.2 kDa.
quantity: 100 µg
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to the supplier - domestic rabbit monoclonal (PSH0-92)
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody (HA721530) at 1/1,000 dilution. Lane 1: 293T over-expressed FUNDC1 cell lysate Lane 2: 293T control cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721530) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FUNDC1 on HepG2 cell lysates with Rabbit anti-FUNDC1 antibody (HA721530) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721530) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-FUNDC1 antibody (HA721530) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721530) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH0-92)
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody (HA750672) at 1/1,000 dilution. Lane 1: 293T over-expressed FUNDC1 cell lysate Lane 2: 293T control cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750672) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FUNDC1 on HepG2 cell lysates with Rabbit anti-FUNDC1 antibody (HA750672) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750672) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-FUNDC1 antibody (HA750672) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750672) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody (ER65664) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: HCT 116 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: RAW264.7 cell lysate (20 µg/Lane) Lane 8: Mouse heart tissue lysate (40 µg/Lane) Lane 9: Mouse liver tissue lysate (40 µg/Lane) Lane 10: Rat liver tissue lysate (40 µg/Lane) Lane 11: Human liver tissue lysate (40 µg/Lane) Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65664) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-FUNDC1 antibody (ER65664) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65664) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Fundc1
- description:FUN14 domain containing 1
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product type
- Fundc1 antibody
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