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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
Figure 1. Western blot analysis of FOXL1 using anti-FOXL1 antibody (A08745-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates,. Lane 2: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXL1 antigen affinity purified polyclonal antibody (Catalog # A08745-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXL1 at approximately 36KD. The expected band size for FOXL1 is at 36KD.
quantity: 100 ug/vial
price: 240 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
gene information - rat Foxl1
- description:forkhead box L1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Foxl1 antibody
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