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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
Western Blot: Factor XII/F12 Antibody [NBP1-94203] - WB analysis of F12 in 1. NIH 3T3 cell lysate and 2. Cos7 cell lysate.
quantity: 0.1 ml (also 0.025 ml)
price: 409 USD
to the supplier - domestic rabbit monoclonal (PSH12-21)
reactivity: mouse, rat
application: ELISA 
Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723443) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723444, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 22,555,940 pg/mL in mouse serum and 20,935 pg/ml in mouse liver Supernatant.
Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit monoclonal (PSH12-23)
reactivity: mouse, rat
application: ELISA 
Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723443) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723444, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 22,555,940 pg/mL in mouse serum and 20,935 pg/ml in mouse liver Supernatant.
Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit monoclonal (PSH12-23)
reactivity: mouse, rat
conjugate: biotin
application: ELISA 
Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723443) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723444, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 22,555,940 pg/mL in mouse serum and 20,935 pg/ml in mouse liver Supernatant.
Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
gene information - rat F12
- synonym:HAF
- description:coagulation factor XII
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- F12 antibody
- F12 ELISA/assay
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