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- mouse monoclonal (7C10)
reactivity: human, mouse, rat
application: western blot, ELISA
citations: 3 - domestic rabbit monoclonal (PSH02-64)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (PSH02-64)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of ERM / Etv5 on different lysates with Rabbit anti-ERM / Etv5 antibody (HA721842) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: HT-29 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: A549 cell lysate Lane 6: HEK-293 cell lysate Lane 7: Hela cell lysate Lane 8: HCT116 cell lysate Lane 9: Neuro-2a cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721842) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ERM / Etv5 antibody (HA721842) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721842) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ERM / Etv5 antibody (HA721842) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721842) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH02-64)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of ERM / Etv5 on different lysates with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: HT-29 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: A549 cell lysate Lane 6: HEK-293 cell lysate Lane 7: Hela cell lysate Lane 8: HCT116 cell lysate Lane 9: Neuro-2a cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750821) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750821) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier - mouse monoclonal (7C10)
reactivity: human, mouse, rat
application: western blot
citations: 1
gene information - rat Etv5
- description:ETS variant transcription factor 5
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Etv5 antibody
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