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- domestic rabbit monoclonal (SC57-07)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of Estrogen Related Receptor alpha on different lysates with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: mouse brain tissue lysate Lane 3: mouse kidney tissue lysate Lane 4: rat brain tissue lysate Lane 5: rat kidney tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of Estrogen Related Receptor alpha was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-14, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (SC57-07)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section 
Western blot analysis of Estrogen Related Receptor alpha on different lysates with Rabbit anti-Estrogen Related Receptor alpha antibody (HA750200) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: mouse brain tissue lysate Lane 3: mouse kidney tissue lysate Lane 4: rat brain tissue lysate Lane 5: rat kidney tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750200) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of Estrogen Related Receptor alpha was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750200, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (HA750200) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750200) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat Esrra
- synonym:ERRalpha; Errra
- description:estrogen related receptor, alpha
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- Esrra antibody
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