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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal (JE64-87)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: Caco-2 cell lysate (20 µg/Lane) Lane 5: HDLM-2 cell lysate (20 µg/Lane) Lane 6: HepG2 cell lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of AER61 on different lysates with Rabbit anti-AER61 antibody (HA721973) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (40 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721973) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - rat Eogt
- synonym:Aer61
- description:EGF domain specific O-linked N-acetylglucosamine transferase
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product type
- Eogt antibody
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