rat Enpp3 antibody | 6 total products from 2 suppliers

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Anti-ENPP3/B10 antibody [EPR27349-87]
Abcam
catalog: ab312316

total number of products: 4

domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

quantity: 20µL, 100µL, 1 mL
price:
to the supplier
Anti-ENPP3/B10 antibody [EPR27349-87] - BSA and Azide free
Abcam
catalog: ab312317

total number of products: 4

domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

quantity: 100µg, 1 mg
price:
to the supplier
Anti-ENPP3/B10 antibody [EPR27349-126]
Abcam
catalog: ab313376

total number of products: 4

domestic rabbit monoclonal
reactivity: human, rat
application: immunohistochemistry - paraffin section

quantity: 20µL, 100µL, 1 mL
price:
to the supplier
ENPP3 / B10
HUABIO
catalog: HA722774

domestic rabbit monoclonal (PSH06-96)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of ENPP3 / B10 on different lysates with Rabbit anti-ENPP3 / B10 antibody (HA722774) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Rat uterus tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722774) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of ENPP3 / B10 on different lysates with Rabbit anti-ENPP3 / B10 antibody (HA722774) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T transfected with ENPP3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 100 kDa Observed band size: 100/130 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722774) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells transfected with or without ENPP3 labeling ENPP3 / B10 with Rabbit anti-ENPP3 / B10 antibody (HA722774) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ENPP3 / B10 antibody (HA722774) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
ENPP3 / B10
HUABIO
catalog: HA751123

domestic rabbit monoclonal (PSH06-96)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of ENPP3 / B10 on different lysates with Rabbit anti-ENPP3 / B10 antibody (HA751123) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Rat uterus tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751123) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of ENPP3 / B10 on different lysates with Rabbit anti-ENPP3 / B10 antibody (HA751123) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T transfected with ENPP3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 100 kDa Observed band size: 100/130 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751123) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells transfected with or without ENPP3 labeling ENPP3 / B10 with Rabbit anti-ENPP3 / B10 antibody (HA751123) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ENPP3 / B10 antibody (HA751123) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier