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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 5 - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of ENAH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500026, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate
Western blot analysis of ENAH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500026, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Rat brain tissue lysate
Immunocytochemistry analysis of MG-63 cells labeling ENAH with Rabbit anti-ENAH antibody (HA500026) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ENAH antibody (HA500026) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
gene information - rat Enah
- description:ENAH, actin regulator
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product type
- Enah antibody
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