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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal (JE37-39)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (HA722546) at 1/1,000 dilution. Lane 1: Human brain tissue lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722546) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Engrailed 1 was immunoprecipitated from 0.2 mg rat brain tissue lysate with HA722546 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722546 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Rat brain tissue lysate (input) Lane 2: HA722546 IP in rat brain tissue lysate Lane 3: Rabbit IgG instead of HA722546 in rat brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 50 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH08-17)
reactivity: human, mouse, rat
application: western blot, flow cytometry 
Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (HA722960) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722960) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of SH-SY5Y cells labeling Engrailed 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722960, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH08-17)
reactivity: human, mouse, rat
application: western blot, flow cytometry 
Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (HA751208) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751208) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of SH-SY5Y cells labeling Engrailed 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751208, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat En1
- description:engrailed homeobox 1
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product type
- En1 antibody
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