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- domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JG34-59)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of ELMO1 on different lysates with Rabbit anti-ELMO1 antibody (ET7108-06) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Raji cell lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 75 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-06) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-ELMO1 antibody (ET7108-06) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ELMO1 antibody (ET7108-06) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat, dogs, bovine, zebrafish
application: western blot, immunoprecipitation, enzyme immunoassay - domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section 
Western Blot: ELMO1 Antibody [NBP1-33645] - ELMO1 antibody detects ELMO1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw 264.7 whole cell lysate/extract. G. 30 µg C2Cl2 whole cell lysate/extract. 7.5% SDS-PAGE. ELMO1 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
quantity: 100 ul
price: 459 USD
to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
gene information - rat Elmo1
- synonym:ELMO1s
- description:engulfment and cell motility 1
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- Elmo1 antibody
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