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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 1
Western blot analysis of mouse brain membranes (lanes 1 and 3) and human Malme-3M melanoma cell lysate (lanes 2 and 4): - 1,2. Anti-Ephrin-A1 (extracellular) Antibody (#AER-031), (1:200).3,4. Anti-Ephrin-A1 (extracellular) Antibody, preincubated with Ephrin-A1 (extracellular) Blocking Peptide (#BLP-ER031).
Expression of Ephrin-A1 in rat PC12 cells - Cell surface detection ofEphrin-A1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-Ephrin-A1 (extracellular) Antibody (#AER-031) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
Western blot analysis of mouse brain membranes (lanes 1 and 3) and human Malme-3M melanoma cell lysate (lanes 2 and 4): - 12.Anti-Ephrin-A1 (extracellular) Antibody (#AER-031) (1:200).34. Anti-Ephrin-A1 (extracellular) Antibody preincubated with the negative control antigen.
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to the supplier - mouse monoclonal (A7B10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Ephrin A1 on HUVEC cell lysates with Mouse anti-Ephrin A1 antibody (HA601038) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601038) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Ephrin A1 on SK-Br-3 cell lysates with Mouse anti-Ephrin A1 antibody (HA601038) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601038) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HUVEC cells labeling Ephrin A1 with Mouse anti-Ephrin A1 antibody (HA601038) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Ephrin A1 antibody (HA601038) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 360.00 USD
to the supplier
gene information - rat Efna1
- synonym:B61
- description:ephrin A1
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product type
- Efna1 antibody
- Efna1 protein
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