E2F2
HUABIO
catalog: ET1611-88

domestic rabbit monoclonal (SN201-04)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of E2F2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate

Flow cytometric analysis of E2F2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-88, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier