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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 4
Western blot analysis of whole rat brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes: - 1,2. Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200).3,4. Anti-D3 Dopamine Receptor (extracellular) Antibody, preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).
Expression of DRD3 in rat striatum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:100). A. DRD3 appears in a subset of striatal neurons (green). B. The same section was stained for parvalbumin (red), a marker of interneurons. C. Merging the two images demonstrates that DRD3 localization includes parvalbuminergic striatal interneurons. DAPI was used as the counterstain (blue).
Peptide CGAENSTGVNRARPH corresponding to amino acid residues 15-29 of rat DRD3(AccessionP19020). Extracellular N-terminus.
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to the supplier - domestic rabbit polyclonal (polyclonal)
reactivity: rat
application: western blot, immunohistochemistry - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, dot blot - domestic rabbit monoclonal (JE33-33)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of Dopamine Receptor D3 on different lysates with Rabbit anti-Dopamine Receptor D3 antibody (HA721391) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: SH-SY5Y cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 44 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721391) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Drd3
- description:dopamine receptor D3
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