RCL
HUABIO
catalog: HA720027

domestic rabbit monoclonal (JE59-29)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of RCL on different lysates with Rabbit anti-RCL antibody (HA720027) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RCL KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720027) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of RCL on PC-12 cell lysates with Rabbit anti-RCL antibody (HA720027) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720027) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of RCL on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720027, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: HepG2 cell lysate
quantity: 100μl
price: 385.00 USD
to the supplier