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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 4 - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - domestic rabbit monoclonal (PSH14-69)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of BDH1 on different lysates with Rabbit anti-BDH1 antibody (HA723667) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: COLO205 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Predicted band size: 38 kDa Observed band size: 33 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723667) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of BDH1 on different lysates with Rabbit anti-BDH1 antibody (HA723667) at 1/20,000 dilution. Lane 1: COS-1 cell lysate (10 µg/Lane) Lane 2: Mouse liver tissue lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (10 µg/Lane) Lane 4: Mouse heart tissue lysate (10 µg/Lane) Lane 5: Rat liver tissue lysate (10 µg/Lane) Lane 6: Rat brain tissue lysate (10 µg/Lane) Lane 7: Rat heart tissue lysate (10 µg/Lane) Predicted band size: 38 kDa Observed band size: 33 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723667) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling BDH1 with Rabbit anti-BDH1 antibody (HA723667) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BDH1 antibody (HA723667) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH14-69)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of BDH1 on different lysates with Rabbit anti-BDH1 antibody (HA751532) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: COLO205 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Predicted band size: 38 kDa Observed band size: 33 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751532) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of BDH1 on different lysates with Rabbit anti-BDH1 antibody (HA751532) at 1/20,000 dilution. Lane 1: COS-1 cell lysate (10 µg/Lane) Lane 2: Mouse liver tissue lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (10 µg/Lane) Lane 4: Mouse heart tissue lysate (10 µg/Lane) Lane 5: Rat liver tissue lysate (10 µg/Lane) Lane 6: Rat brain tissue lysate (10 µg/Lane) Lane 7: Rat heart tissue lysate (10 µg/Lane) Predicted band size: 38 kDa Observed band size: 33 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751532) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling BDH1 with Rabbit anti-BDH1 antibody (HA751532) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BDH1 antibody (HA751532) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat D beta hydroxybutyrate dehydrogenase
- synonym:Bdh
- description:3-hydroxybutyrate dehydrogenase 1
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product type
- D beta hydroxybutyrate dehydrogenase antibody
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