Your Filters
product
- domestic rabbit polyclonal
reactivity: human, rat
application: western blot, flow cytometry
Western blot analysis of CYP7A1 on different lysates with Rabbit anti-CYP7A1 antibody (HA500207) at 1/1,000 dilution. Lane 1: LO2 cell lysate Lane 2: Huh7 cell lysate Lane 3: Hepa1-6 cell lysate Lane 4: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of SiHa cells labeling CYP7A1 with Rabbit anti-CYP7A1 antibody (HA500207) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CYP7A1 antibody (HA500207) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of CYP7A1 was done on HeLa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500207, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Cyp7a1
- synonym:CHAP; CYP7; CYP7S1
- description:cytochrome P450 family 7 subfamily A member 1
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
linkout
questions and comments