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- domestic rabbit monoclonal
reactivity: mouse, rat
application: western blot, immunocytochemistry - domestic rabbit monoclonal (HL1629)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry - domestic rabbit monoclonal (JE75-34)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of Delta-catenin on different lysates with Rabbit anti-Delta-catenin antibody (HA722438) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Mouse heart tissue lysate (negative) Lane 4: Mouse kidney tissue lysate (negative) Lane 5: Mouse cerebellum tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat heart tissue lysate (negative) Lane 8: Rat kidney tissue lysate (negative) Lane 9: Rat spleen tissue lysate (negative) Lane 10: Rat hippocampus tissue lysate Lane 11: Rat cerebellum tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 135 kDa Observed band size: 135 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722438) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Delta-catenin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722438 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722438 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA722438 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA722438 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds; ECL: K1801
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of Delta-catenin on different lysates with Rabbit anti-Delta-catenin antibody (HA500428) at 1/500 dilution. Lane 1: Human brain tissue lysate(20 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500428) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 30 minutes at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Ctnnd2
- description:catenin delta 2
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product type
- Ctnnd2 antibody
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