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- domestic rabbit monoclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal (8F1U7)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE55-32)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CTNNA3 on different lysates with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/1,000 dilution. Lane 1: Rat heart tissue lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (20 µg/Lane) Lane 3: 293 cell lysate (10 µg/Lane) Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-07) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-CTNNA3 antibody (ET7111-07) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - rat Ctnna3
- synonym:Catna3; RGD1559455; RGD1562230
- description:catenin alpha 3
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Ctnna3 antibody
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