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- domestic rabbit monoclonal (JG39-73)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of CtBP1 on different lysates with Rabbit anti-CtBP1 antibody (ET7108-96) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CtBP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-96) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CtBP1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-96, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 47 kDa Observed band size: 47 kDa
ICC staining of CtBP1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-96, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, immunocytochemistry - mouse monoclonal (3/CtBP1)
reactivity: human, mouse, rat, dogs, chicken
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, other
citations: 15
gene information - rat Ctbp1
- synonym:50-kDaBFA-inducedADP-ribosylatedsubstrate; BARS-50; Bars; CtBP3/BARS
- description:C-terminal binding protein 1
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product type
- Ctbp1 antibody
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