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- domestic rabbit monoclonal (SD83-03)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Collagen VI on different lysates with Rabbit anti-Collagen VI antibody (ET1612-91) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: Human kidney tissue lysate (20 µg/Lane) Lane 3: Rat heart tissue lysate (20 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 109 kDa Observed band size: 145 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-91) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Collagen VI on different lysates with Rabbit anti-Collagen VI antibody (ET1612-91) at 1/1,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 145 kDa Observed band size: 145 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-91) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of Collagen VI in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 50μl
price: 205.00 USD
to the supplier - domestic rabbit monoclonal (SD83-03)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Collagen VI on different lysates with Rabbit anti-Collagen VI antibody (HA750305) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: Human kidney tissue lysate (20 µg/Lane) Lane 3: Rat heart tissue lysate (20 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 109 kDa Observed band size: 145 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Collagen VI on different lysates with Rabbit anti-Collagen VI antibody (HA750305) at 1/1,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 145 kDa Observed band size: 145 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750305) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of Collagen VI in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750305, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit monoclonal (SD83-03)
reactivity: human, mouse, rat 
Immunocytochemistry analysis of Hela cells labeling Collagen VI with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI (HA720155F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Collagen VI (HA720155F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
quantity: 100μl
price: 429.00 USD
to the supplier
gene information - rat Col6a3
- description:collagen type VI alpha 3 chain
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
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product type
- Col6a3 antibody
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