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- domestic rabbit monoclonal (JB42-04)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of KDEL on different lysates with Rabbit anti-KDEL antibody (ET7107-86) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: AGS cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Rat placenta tissue lysate (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 50-100 kDa Exposure time: Lane 1-4: 1 minute; Lane 5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of KDEL on different lysates with Rabbit anti-KDEL antibody (ET7107-86) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse placenta (E16.5) tissue lysate (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 50-100 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of KDEL on different lysates with Rabbit anti-KDEL antibody (ET7107-86) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Mouse colon tissue lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Lane 4: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 50-100 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
gene information - rat Col4a3
- description:collagen type IV alpha 3 chain
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Col4a3 antibody
- Col4a3 protein
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