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- domestic rabbit monoclonal (SR46-05)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (ET1602-14) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: Mouse skin tissue lysate (20 µg/Lane) Lane 3: Rat skin tissue lysate (20 µg/Lane) Predicted band size: 148 kDa Observed band size: 130、180 kDa Exposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (ET1602-14) at 1/1,000 dilution. Lane 1: Wild-type caski whole cell lysate (10 µg). Lane 2/3: Collagen XVII knockdown caski whole cell lysate (10 µg). Predicted band size: 148 kDa Observed band size: 130、180 kDa Exposure time: 1 minutes 40 seconds; ECL: K1801;4-20% SDS-PAGE gel. ET1602-14 was shown to specifically react with Collagen XVII in wild-type caski cells. Weakened bands were observed when Collagen XVII knockdown samples were tested. Wild-type and Collagen XVII knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-14) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen XVII antibody (ET1602-14) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-14) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier - domestic rabbit monoclonal (SR46-05)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section 
Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (HA750030) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: Mouse skin tissue lysate (20 µg/Lane) Lane 3: Rat skin tissue lysate (20 µg/Lane) Predicted band size: 148 kDa Observed band size: 130、180 kDa Exposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750030) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (HA750030) at 1/1,000 dilution. Lane 1: Wild-type caski whole cell lysate (10 µg). Lane 2/3: Collagen XVII knockdown caski whole cell lysate (10 µg). Predicted band size: 148 kDa Observed band size: 130、180 kDa Exposure time: 1 minutes 40 seconds; ECL: K1801;4-20% SDS-PAGE gel. HA750030 was shown to specifically react with Collagen XVII in wild-type caski cells. Weakened bands were observed when Collagen XVII knockdown samples were tested. Wild-type and Collagen XVII knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA750030) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen XVII antibody (HA750030) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat Col17a1
- description:collagen type XVII alpha 1 chain
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- Col17a1 antibody
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