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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of CNOT1 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500466, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of CNOT1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500466, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay - Rabbit anti-human CCR4-NOT transcription complex, subunit 1 polyclonal Antibody
MyBioSource
catalog: MBS711096domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, enzyme immunoassay
gene information - rat Cnot1
- synonym:RGD1308009
- description:CCR4-NOT transcription complex, subunit 1
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product type
- Cnot1 antibody
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