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- domestic rabbit polyclonal (NA)
reactivity: mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of rat brain lysate: - 1. Anti-CNGA4 Antibody (#APC-074), (1:200).2. Anti-CNGA4 Antibody, preincubated with CNGA4 Blocking Peptide (#BLP-PC074).
Expression of CNGA4 in mouse olfactory bulb - Immunohistochemical staining of CNGA4 channel in mouse olfactory bulb using Anti-CNGA4 Antibody (#APC-074). A. CNGA4 staining (green) appears in glomeruli (horizontal arrows) and outside the glomeruli (vertical arrows). To define the location of glomeruli (G), the same section was stained with mouse anti-synaptophysin (red) and the nissl stain DAPI (blue). B. Pre-incubation of Anti-CNGA4 Antibody with the peptide antigen blocks the staining in the olfactory bulb.
Expression of CNGA4 in mouse olfactory bulb - Immunohistochemical staining of CNGA4 channel in mouse olfactory bulb usingAnti-CNGA4 Antibody (#APC-074). A. CNGA4 staining (green) appears in glomeruli (horizontal arrows) and outside the glomeruli (vertical arrows). To define the location of glomeruli (G) the same section was stained with mouse anti-synaptophysin (red) and the nissl stain DAPI (blue). B. Pre-incubation of Anti-CNGA4 Antibody with the peptide antigen blocks the staining in the olfactory bulb.
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry 
Western blot analysis of CNGA4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: A431 cell lysate
Western blot analysis of CNGA4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human brain tissue lysate Lane 2: SHSY5Y cell lysate
ICC staining of CNGA4 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-88, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal (polyclonal)
reactivity: rat
application: western blot, immunohistochemistry
gene information - rat Cnga4
- synonym:Cgn2; rOCNC2
- description:cyclic nucleotide gated channel subunit alpha 4
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product type
- Cnga4 antibody
- Cnga4 protein
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