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- domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1 - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (ER1902-48) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CLIC4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-48) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CLIC4 on rat kidney (1) and mouse kidney (2) tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CLIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-48, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (JE65-09)
reactivity: human, mouse, rat
application: western blot, flow cytometry 
Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (HA722058) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: JAR cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: Neuro-2a cell lysate (20 µg/Lane) Lane 6: C6 cell lysate (20 µg/Lane) Lane 7: Human kidney tissue lysate (40 µg/Lane) Lane 8: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722058) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (HA722058) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CLIC4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722058) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling CLIC4 with Rabbit anti-CLIC4 antibody (HA722058) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLIC4 antibody (HA722058) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry 
Western blot analysis of human pancreatic carcinoma PANC-1 (lanes 1 and 4), mouse kidney (lanes 2 and 5) and rat kidney (lanes 3 and 6) lysates: - 1-3. Anti-CLIC4 Antibody (#ACL-024), (1:200).4-6. Anti-CLIC4 Antibody, preincubated with CLIC4 Blocking Peptide (#BLP-CL024).
Peptide (C)NGLKEEDKEPLIE corresponding to amino acid residues 8-20 of human CLIC4 (AccessionQ9Y696).Intracellular N-terminus.
Expression of CLIC4 in human pancreatic carcinoma PANC-1 cells - Immunocytochemical staining of fixed and permeabilized human pancreatic carcinoma PANC-1 cells. A. Cells were stained withAnti-CLIC4 Antibody(#ACL-024) (1:200) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B.Cell nuclei were visualized using Hoechst 33342 (blue).C. Live image of the cells.
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gene information - rat Clic4
- description:chloride intracellular channel 4
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product type
- Clic4 antibody
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