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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of LI Cadherin on different lysates with Rabbit anti-LI Cadherin antibody (ER1804-02) at 1/5,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: LoVo cell lysate Lane 3: PANC-1 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 120 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1804-02) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-LI Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1804-02) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-LI Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1804-02) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - Cadherin-17 / LI-cadherin / CDH17 Antibody, Rabbit PAb, Antigen Affinity Purifie ...
Sino Biological
catalog: 80283-T16domestic rabbit polyclonal
reactivity: rat
application: ELISA
gene information - rat Cdh17
- description:cadherin 17
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product type
- Cdh17 antibody
- Cdh17 protein
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