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- rat monoclonal (191704)
reactivity: human, rat
conjugate: APC
application: immunohistochemistry, immunocytochemistry, flow cytometry
citations: 6
Detection of CCR8 antibody in Human Blood Monocytes antibody by Flow Cytometry.
quantity: 100 Tests (also 25 Tests)
price: 469 USD
to the supplier - rat monoclonal (191704)
reactivity: human, rat
conjugate: PE
application: flow cytometry
citations: 5 
Detection of CCR8 antibody in Human PBMCs antibody by Flow Cytometry.
quantity: 25 Tests (also 100 Tests)
price: 159 USD
to the supplier - domestic sheep polyclonal
reactivity: mouse, rat
application: western blot 
Detection of Mouse and Rat CCR8 antibody by Western Blot.
quantity: 100 ug (also 25 ug)
price: 489 USD
to the supplier - domestic rabbit monoclonal (JE31-99)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of CCR8 on mouse thymus tissue lysates with Rabbit anti-CCR8 antibody (HA721360) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 41 kDa Observed band size: 48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721360) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CCR8 on rat thymus tissue lysates with Rabbit anti-CCR8 antibody (HA721360) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 41 kDa Observed band size: 48 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721360) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Raji cells labeling CCR8 with Rabbit anti-CCR8 antibody (HA721360) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody (HA721360) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Ccr8
- description:C-C motif chemokine receptor 8
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Ccr8 antibody
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