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- domestic rabbit polyclonal (JM10-35)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2 - domestic rabbit monoclonal (JE55-84)
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CARS on different lysates with Rabbit anti-CARS antibody (ET7111-18) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 85 kDa Observed band size: 75 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-18) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CARS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-CARS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section
gene information - rat Cars
- description:cysteinyl-tRNA synthetase
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- Cars antibody
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