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- domestic rabbit polyclonal
reactivity: human, rat
application: western blot, flow cytometry
Western blot analysis of ATP8A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Rat brain tissue lysate
Flow cytometric analysis of ATP8A1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-61, 1/50) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple).
quantity: 100μl
price: 330 USD
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gene information - rat Atp8a1
- description:ATPase phospholipid transporting 8A1
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product type
- Atp8a1 antibody
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