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- domestic rabbit monoclonal (JE34-00)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of ATP1A2 on different lysates with Rabbit anti-ATP1A2 antibody (HA721422) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 112 kDa Observed band size: 100 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721422) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-ATP1A2 antibody (HA721422) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721422) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-ATP1A2 antibody (HA721422) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721422) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JE34-00)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002), (1:200).3,4. Anti-Alpha 2 Na+/K+ ATPase Antibody, preincubated with Alpha 2 Na+/K+ ATPase Blocking Peptide (#BLP-NP002).
Expression of Na+/K+ ATPase α2 (ATP1A2) in mouse hippocampus and cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. A. ATP1A2 staining (green) in mouse hippocampal CA3 region is labeled in astrocytic processes (arrows surrounding pyramidal cells, P) and diffused staining is observed in the CA3 stratum radiatum. Note absence of staining in the mossy fiber terminal region (MF). B. Staining in mouse cerebellum shows labeling of the basket shape (arrows) under Purkinje cells (P) and diffused staining in the molecular layer (MOL). Cell nuclei are stained with DAPI (blue).
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to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot
gene information - rat Atp1a2
- synonym:RATATPA2
- description:ATPase Na+/K+ transporting subunit alpha 2
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Atp1a2 antibody
- Atp1a2 protein
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