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- domestic rabbit monoclonal (SN73-07)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of ATG5 on different lysates with Rabbit anti-ATG5 antibody (ET1611-38) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: C2C12 cell lysate (15 µg/Lane) Lane 3: Neuro-2a cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (15 µg/Lane) Lane 6: Rat brain tissue lysate (15 µg/Lane) Predicted band size: 32 kDa Observed band size: 55 kDa Exposure time: 35 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-38) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATG5 on different lysates with Rabbit anti-ATG5 antibody (ET1611-38) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Neuro-2a cell lysate (20 µg/Lane) Predicted band size: 32 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-38) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATG5 with anti-ATG5 antibody [SN73-07] (ET1611-38) at 1/1,000 dilution. Lane 1: Wild-type Huh7 whole cell lysate. Lane 2: ATG5 knockout Huh7 whole cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ATG5 antibody (ET1611-38, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (SN73-07)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of ATG5 on different lysates with Rabbit anti-ATG5 antibody (HA750251) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: C2C12 cell lysate (15 µg/Lane) Lane 3: Neuro-2a cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (15 µg/Lane) Lane 6: Rat brain tissue lysate (15 µg/Lane) Predicted band size: 32 kDa Observed band size: 55 kDa Exposure time: 35 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750251) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATG5 on different lysates with Rabbit anti-ATG5 antibody (HA750251) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Neuro-2a cell lysate (20 µg/Lane) Predicted band size: 32 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750251) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATG5 with anti-ATG5 antibody [SN73-07] (HA750251) at 1/1,000 dilution. Lane 1: Wild-type Huh7 whole cell lysate. Lane 2: ATG5 knockout Huh7 whole cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ATG5 antibody (ET1611-38, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit monoclonal (SN73-07)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section - mouse monoclonal (ATG5-18)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, western blot knockout validation
citations: 8
gene information - rat Atg5
- description:autophagy related 5
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Atg5 antibody
- Atg5 ELISA/assay
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