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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry - domestic rabbit monoclonal (PSH05-23)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of ATG4C on different lysates with Rabbit anti-ATG4C antibody (HA722252) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Raji cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HeLa cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: HL-60 cell lysate (20 µg/Lane) Lane 8: Mouse brain tissue lysate (40 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Lane 10: COS-1 cell lysate (20 µg/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722252) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ATG4C was immunoprecipitated from 0.2 mg Raji cell lysate with HA722252 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722252 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA722252 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA722252 in Raji cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 21 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH05-23)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation 
Western blot analysis of ATG4C on different lysates with Rabbit anti-ATG4C antibody (HA750994) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Raji cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HeLa cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: HL-60 cell lysate (20 µg/Lane) Lane 8: Mouse brain tissue lysate (40 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Lane 10: COS-1 cell lysate (20 µg/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750994) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ATG4C was immunoprecipitated from 0.2 mg Raji cell lysate with HA750994 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750994 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA750994 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA750994 in Raji cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 21 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
gene information - rat Atg4c
- description:autophagy related 4C, cysteine peptidase
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product type
- Atg4c antibody
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