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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of mouse ND7/23 neuroblastoma x rat DRG neuron hybrid cell line (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates: - 1,2. Anti-ASIC4 Antibody (#ASC-015), (1:200).3,4. Anti-ASIC4 Antibody, preincubated with ASIC4 Blocking Peptide (#BLP-SC015).
Western blot analysisof ND7/23 cell line (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates: - 12.Anti-ASIC4Antibody (#ASC-015) (1:200).34. Anti-ASIC4 Antibody preincubated with the negative control antigen.
Peptide CKIKFAEEDAKPKEKEAGDE corresponding to amino acid residues 7-26 of rat ASIC4 (AccessionQ9QYV9).Intracellular N-terminus.
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of ASIC4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500151, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Human brain tissue lysate Lane 3: K562 cell lysate
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ASIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500151, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue using anti-ASIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500151, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal (polyclonal)
reactivity: rat
application: western blot
gene information - rat Asic4
- synonym:Accn4; Spasic
- description:acid sensing ion channel subunit family member 4
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Asic4 antibody
- Asic4 protein
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