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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE54-62)
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (ET7110-78) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD APLP2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 2 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-78) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (ET7110-78) at 1/500 dilution. Lane 1: Rat brian tissue lysate(20 µg/Lane) Lane 2: Human placenta tissue lysate(20 µg/Lane) Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 100/120 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-78) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-APLP2 antibody (ET7110-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - rat Aplp2
- synonym:APLP-2; Apph; WALPLP2
- description:amyloid beta precursor like protein 2
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product type
- Aplp2 antibody
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