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- domestic rabbit polyclonal (NA)
reactivity: mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 5
Western blot analysis of rat dorsal root ganglion (DRG) lysate: - 1. Anti-TMEM16A (ANO1) (extracellular) Antibody (#ACL-011), (1:200).2. Anti-TMEM16A (ANO1) (extracellular) Antibody, preincubated with TMEM16A/ANO1 (extracellular) Blocking Peptide (#BLP-CL011).
Western blot analysis of rat small intestine lysate: - 1.Anti-TMEM16A (ANO1) (extracellular)Antibody (#ACL-011) (1:200).2. Anti-TMEM16A (ANO1) (extracellular) Antibody preincubated with the control peptide antigen.
Peptide (C)EYVKRKQRYEVDFNLE corresponding to amino acid residues 679-694 of mouse Anoctamin-1 (AccessionQ8BHY3). 3rdextracellular loop.
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to the supplier - mouse monoclonal (OTI1C8)
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section - mouse monoclonal (OTI1C8)
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal (PSH06-57)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of TMEM16A on different lysates with Rabbit anti-TMEM16A antibody (HA722719) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HCT 116 cell lysate Lane 3: BxPC-3 cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 114 kDa Observed band size: 150 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722719) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue with Rabbit anti-TMEM16A antibody (HA722719) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722719) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-TMEM16A antibody (HA722719) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722719) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Ano1
- synonym:Tmem16a
- description:anoctamin 1
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product type
- Ano1 antibody
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