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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
Western Blot: AMID Antibody - BSA Free [NBP3-18133] - WB Validation in Mouse Skeletal Muscle. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18133, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.
quantity: 0.1 mg
price: 409 USD
to the supplier - domestic rabbit monoclonal (PSH15-60)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of AMID on different lysates with Rabbit anti-AMID antibody (HA723767) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (no heat) (15 µg/Lane) Lane 3: HeLa cell lysate (no heat) (20 µg/Lane) Lane 4: NCI-H1299 cell lysate (no heat) (20 µg/Lane) Lane 5: LO2 cell lysate (15 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 41 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723767) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling AMID with Rabbit anti-AMID antibody (HA723767) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMID antibody (HA723767) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Application: IF-Tissue Species: Rat Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/200
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH15-60)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of AMID on different lysates with Rabbit anti-AMID antibody (HA751571) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (no heat) (15 µg/Lane) Lane 3: HeLa cell lysate (no heat) (20 µg/Lane) Lane 4: NCI-H1299 cell lysate (no heat) (20 µg/Lane) Lane 5: LO2 cell lysate (15 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 41 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751571) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling AMID with Rabbit anti-AMID antibody (HA751571) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMID antibody (HA751571) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Application: IF-Tissue Species: Rat Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/200
quantity: 100μl
price: 385.00 USD
to the supplier - mouse monoclonal (Pab122)
reactivity: human, mouse, rat
application: immunocytochemistry, immunoprecipitation, flow cytometry, FACS
gene information - rat Aifm2
- synonym:Amid; Prg3
- description:apoptosis inducing factor, mitochondria associated 2
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Aifm2 antibody
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