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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of rat brain stem (lanes 1 and 3), mouse brain (lanes 2 and 4), SH-SY5Y (lanes 5 and 7) and Jurkat (lanes 6 and 8) lysates: - 1,2,5,6. Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:200).3,4,7,8. Anti-α1D-Adrenergic Receptor (extracellular) Antibody, preincubated with α1D-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR019).
Expression of α1D-Adrenoceptor in rat heart - Immunohistochemical staining of rat heart paraffin embedded sections using Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:100). α1D-Adrenoceptor is expressed in cardiomyocytes of the myocardium and in the smooth muscle of the blood vessels (arrows). Hematoxilin is used as the counterstain.
Cell surface detection of ?1D-Adrenoceptorin intact live Jurkat cells: - ___ Unstained cells.___ Cells + Anti-?1D-Adrenergic Receptor (extracellular)Antibody (#AAR-019) (5-10 g antibody/0.5x106 cells).
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to the supplier - unidentified polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis of Alpha-1D Adrenergic Receptor expression in MCF7 (A), RAW264.7 (B), H9C2 (C) whole cell lysates.
Immunohistochemical analysis of Alpha-1D Adrenergic Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Alpha-1D Adrenergic Receptor staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity: 0.03 mL
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot - domestic rabbit polyclonal (OTI4H2)
reactivity: human, rat, bovine
application: immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal (polyclonal)
reactivity: rat
application: western blot, immunohistochemistry, flow cytometry
gene information - rat Adra1d
- synonym:Adrd1
- description:adrenoceptor alpha 1D
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Adra1d antibody
- Adra1d protein
- Adra1d inhibitor/activator/substrate/reporter
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