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- domestic rabbit polyclonal (Poly28635)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JG82-31)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysates with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size:40 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-64) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling Alcohol Dehydrogenase with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - rat Adh1
- synonym:Adh; Adh1a; Adh1c
- description:alcohol dehydrogenase 1 (class I)
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- Adh1 antibody
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