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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 6 - domestic rabbit monoclonal (JE63-98)
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (HA722071) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722071) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (HA722071) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ACO1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722071) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HeLa cells labeling ACO1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722071, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (R1512-16) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-16) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining ACO1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ACO1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, enzyme immunoassay
gene information - rat Aco1
- synonym:AH; Acon1; IRP1
- description:aconitase 1
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product type
- Aco1 antibody
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