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- domestic goat polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, immunocytochemistry, enzyme immunoassay - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE64-40)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ACAT-1 on different lysates with Rabbit anti-ACAT-1 antibody (HA721093) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACAT-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721093) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ACAT-1 on different lysates with Rabbit anti-ACAT-1 antibody (HA721093) at 1/500 dilution. Lane 1: Raji cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 4 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721093) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ACAT-1 on different lysates with Rabbit anti-ACAT-1 antibody (HA721093) at 1/500 dilution. Lane 1: Rat liver tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 1 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721093) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
gene information - rat Acat1
- synonym:RATACAL
- description:acetyl-CoA acetyltransferase 1
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product type
- Acat1 antibody
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