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- domestic rabbit monoclonal (JE39-25)
reactivity: human, rat
application: western blot, immunoprecipitation
Western blot analysis of ABO on different lysates with Rabbit anti-ABO antibody (HA722455) at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722455) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ABO was immunoprecipitated from 0.2 mg 293T cell lysate with HA722455 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722455 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cell lysate (input) Lane 2: HA722455 IP in 293T cell lysate Lane 3: Rabbit IgG instead of HA722455 in 293T cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 2 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
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gene information - rat Abo3
- synonym:Abo; Abol1; Gbgt1
- description:histo-blood group ABO system transferase 3
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product type
- Abo3 antibody
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