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- mouse monoclonal (ZET)
reactivity: human, mouse, rat
application: flow cytometry
citations: 11 - domestic rabbit monoclonal
reactivity: human, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (SN07-12)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of ABCF1 on K-562 cell lysates with Rabbit anti-ABCF1 antibody (ET1611-63) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 96 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-63) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of ABCF1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-63, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of ABCF1 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-63, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
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gene information - rat Abcf1
- synonym:Abc50
- description:ATP binding cassette subfamily F member 1
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product type
- Abcf1 antibody
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