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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit polyclonal (NA)
reactivity: mouse, rat
application: western blot - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
Figure 1. Western blot analysis of ABCB4 using anti-ABCB4 antibody (MBS177897). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate,. Lane 2: SW620 Whole Cell Lysate,. Lane 3: 22RV1 Whole Cell Lysate,. Lane 4: SKOV Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 142KD. The expected band size for ABCB4 is at 142KD.
Figure 2. IHC analysis of ABCB4 using anti-ABCB4 antibody (MBS177897). ABCB4 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB4 Antibody (MBS177897) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 3. IHC analysis of ABCB4 using anti-ABCB4 antibody (MBS177897). ABCB4 was detected in paraffin-embedded section of Rat Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB4 Antibody (MBS177897) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
quantity: 0.1 mg
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gene information - rat Abcb4
- synonym:Mdr2; Pgy3
- description:ATP binding cassette subfamily B member 4
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product type
- Abcb4 antibody
- Abcb4 protein
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