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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE64-70)
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/2,000 dilution. Lane 1: HepG2 cell lysate, 10 µg/Lane Lane 2: 293T cell lysate, 10 µg/Lane Lane 3: Rat testis tissue lysate, 20 µg/Lane Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/500 dilution. Lane 1: MCF-7 cell lysate, 10 µg/Lane Lane 2: HUVEC cell lysate, 10 µg/Lane Lane 3: NIH/3T3 cell lysate, 10 µg/Lane Lane 4: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HepG2 cells labeling AAMP. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721054, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
gene information - rat Aamp
- description:angio-associated, migratory cell protein
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Aamp antibody
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