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- domestic rabbit polyclonal (L5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation
citations: 83 - domestic rabbit monoclonal (PSH02-40)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal (PSH02-40)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: K-562 cell lysate Lane 6: A549 cell lysate Lane 7: MCF7 cell lysate Lane 8: SW480 cell lysate Lane 9: THP-1 cell lysate Lane 10: SK-OV-3 cell lysate Lane 11: U-2 OS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C2C12 cell lysate Lane 4: bEnd.3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: F9 cell lysate Lane 7: Neuro-2a cell lysate Lane 8: C6 cell lysate Lane 9: L6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Mouse thymus tissue lysate Lane 12: Rat thymus tissue lysate Lane 13: Mouse testis tissue lysate Lane 14: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH02-40)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA750805) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: K-562 cell lysate Lane 6: A549 cell lysate Lane 7: MCF7 cell lysate Lane 8: SW480 cell lysate Lane 9: THP-1 cell lysate Lane 10: SK-OV-3 cell lysate Lane 11: U-2 OS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750805) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (HA750805) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750805) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA750805) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C2C12 cell lysate Lane 4: bEnd.3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: F9 cell lysate Lane 7: Neuro-2a cell lysate Lane 8: C6 cell lysate Lane 9: L6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Mouse thymus tissue lysate Lane 12: Rat thymus tissue lysate Lane 13: Mouse testis tissue lysate Lane 14: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat 8 oxo dGTPase
- synonym:Mth1
- description:nudix hydrolase 1
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product type
- 8 oxo dGTPase antibody
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