rabbit ALDOA antibody | 16 total products from 5 suppliers

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Anti-Aldolase antibody
Abcam
catalog: ab78339

goat polyclonal
reactivity: human, rabbit
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 50 µg
price:
to the supplier
Anti-Aldolase antibody
Abcam
catalog: ab232786

rabbit polyclonal
reactivity: human, mouse, rat, chimpanzee, rabbit
application: western blot, immunohistochemistry - paraffin section

quantity: 100 µg
price:
to the supplier
ALDOA polyclonal antibody
Abnova
catalog: PAB11351

goat polyclonal
reactivity: rabbit
application: western blot, ELISA

quantity: 100 ug
price:
to the supplier
ALDOA polyclonal antibody
Abnova
catalog: PAB28056

goat polyclonal
reactivity: rabbit
application: western blot, ELISA, immunoprecipitation

quantity: 100 ug
price:
to the supplier
Aldolase antibody
Biorbyt
catalog: orb344330

total number of products: 7

goat polyclonal
reactivity: rabbit

Western blot analysis of 300 ul aliquots of whole anti-aldolase antiserum (100-1141) were used to precipitate varying amounts of purified aldolase and precipitates with controls using Aldolase antibody

Immunoprecipitation analysis of was performed with 300 ul of anti aldolase antiserum and an equal volume of varied amounts using Aldolase antibody

Western blot analysis of whole cell lysate prepared from human derived A293 cells using Aldolase antibody
quantity: 100 ug
price: 360 USD
to the supplier
Aldolase antibody
Biorbyt
catalog: orb343922

total number of products: 7

goat polyclonal
reactivity: rabbit

Immunoprecipitation analysis of was performed with 300 ul of anti aldolase antiserum and an equal volume of varied amounts using Aldolase antibody

Western blot analysis of 300 ul aliquots of whole anti-aldolase antiserum (100-1141) were used to precipitate varying amounts of purified aldolase and precipitates with controls using Aldolase antibody

Western blot analysis of whole cell lysate prepared from human derived A293 cells using Aldolase antibody
quantity: 2 ml
price: 1005 USD
to the supplier
Aldolase antibody
Biorbyt
catalog: orb344331

total number of products: 7

goat polyclonal
reactivity: rabbit

Western blot analysis of Hela lysate (Lane1), HEK293 lysate (Lane2), Jurkat lysate (Lane3) using Aldolase antibody

Western blot analysis of 300 ul aliquots of whole anti-aldolase antiserum (100-1141) were used to precipitate varying amounts of purified aldolase and precipitates with controls using Aldolase antibody

Immunoprecipitation analysis of was performed with 300 ul of anti aldolase antiserum and an equal volume of varied amounts using Aldolase antibody
quantity: 25 ul
price: 195 USD
to the supplier
Aldolase Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 200-1641-0100

total number of products: 4

goat polyclonal
reactivity: rabbit
conjugate: biotin
application: western blot, ELISA, immunoprecipitation

Anti aldolase antibody – Immunoprecipitation and Western Blot. 300 µl aliquots of whole anti-aldolase antiserum (100-1141) were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added. The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel. Gel was stained, destained and imaged(see attached) using standard protocols. Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase. Additional higher and lower molecular weight bands correspond to serum proteins.

Anti aldolase antibody– Immunoprecipitation- Immunoprecipitation was performed with 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 mg/ml) of purified aldolase in PBS. Antibody/Antigen mixture was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added. The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel. Gel was stained, destained and imaged(see attached) using standard protocols. Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase. Additional higher and lower molecular weight bands correspond to serum proteins.
quantity: 100 µg
price: 377 USD
to the supplier
Aldolase Antibody
Rockland Immunochemicals
catalog: 100-1141

total number of products: 4

goat polyclonal
reactivity: rabbit
application: western blot, ELISA, immunoprecipitation

Anti aldolase antibody – immunoprecipitation and western blot. 300 µl aliquots of whole anti-aldolase antiserum (100-1141) were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added. The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel. Gel was stained, destained and imaged(see attached) using standard protocols. Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase. Additional higher and lower molecular weight bands correspond to serum proteins.
quantity: 2 mL
price: 1049 USD
to the supplier
Aldolase Antibody
Rockland Immunochemicals
catalog: 200-1141-0100

total number of products: 4

goat polyclonal
reactivity: rabbit
application: western blot, ELISA, immunoprecipitation

IgG purified antibody to rabbit muscle aldolase (100-1141, 200-1141 and 200-1341) was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.
quantity: 100 µg
price: 369 USD
to the supplier
Aldolase A Polyclonal Antibody, HRP
Invitrogen
catalog: PA1-27236

goat polyclonal
reactivity: rabbit
conjugate: HRP
application: western blot, ELISA, immunoprecipitation

quantity: 1 mg
price: 330 USD
to the supplier