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- mouse monoclonal (19F1)
reactivity: human, mouse, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 12
Immunohistochemistry was performed on biopsies of deparaffinized Human skin tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Adiponectin (MA1-054) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100µg
price: 381 USD
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gene information - rabbit ADIPOQ
- synonym:adiponectin; adipocyte complement-related protein of 30 kDa
- description:adiponectin, C1Q and collagen domain containing
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product type
- ADIPOQ antibody
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