NFkB p65 Polyclonal Antibody
Invitrogen
catalog: PA5-16545

rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat, dog, cow, pig, zebrafish , platanna, rabbit
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 20

Immunofluorescent analysis of NFkappaB/p65 (green) in HeLa cells either left untreated (left panel) or treated with 25ng/ml of TNF-alpha (right panel) for 25 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a NFkappaB/p65 polyclonal antibody (Product # PA5-16545) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

Formalin-fixed, paraffin-embedded human placenta stained with NFK/P65 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts,fibroblasts and endothelial cells.

A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus RELA siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.
quantity: 500µL
price: US 349.00
to the supplier